2I4N
Rhodopseudomonas palustris prolyl-tRNA synthetase in complex with CysAMS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-30 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97935 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 217.900, 107.470, 110.660 |
| Unit cell angles | 90.00, 120.43, 90.00 |
Refinement procedure
| Resolution | ? - 2.850 |
| R-factor | 0.21436 |
| Rwork | 0.211 |
| R-free | 0.27676 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.531 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Number of reflections | 48377 | |
| Completeness [%] | 93.5 | 75.5 |
| Redundancy | 2.5 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1 M citric acid (pH 5.5), 10-11 % PEG 3000, 15-20 % ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
