2I4M
Rhodopseudomonas palustris prolyl-tRNA synthetase in complex with ProAMS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-31 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9310 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 110.360, 211.900, 150.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 2.800 |
| R-factor | 0.20932 |
| Rwork | 0.206 |
| R-free | 0.26938 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.419 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.040 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 43346 | |
| Completeness [%] | 96.2 | 91.6 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1 M Citric acid pH 5.5, 10-11 % PEG 3000, 15-20 % ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
