2I4C
Crystal structure of Bicarbonate Transport Protein CmpA from Synechocystis sp. PCC 6803 in complex with bicarbonate and calcium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-30 |
| Detector | Bruker Platinum 135 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.240, 49.240, 57.200 |
| Unit cell angles | 87.40, 80.80, 75.40 |
Refinement procedure
| Resolution | 56.820 - 1.700 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 2I48 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.303 |
| Data reduction software | SAINT |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.820 | 1.780 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 46348 | |
| <I/σ(I)> | 14.3 | 3.5 |
| Completeness [%] | 91.8 | 79.1 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch/microseeding | 8 | 298 | Ammonium sulfate, HEPPS, pH 8.0, batch/microseeding, temperature 298K |
