2I48
Crystal structure of Bicarbonate Transport Protein CmpA from Synechocystis sp. PCC 6803 in complex with carbonic acid
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-03 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97899, 0.97915, 0.97132 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.540, 49.640, 57.780 |
| Unit cell angles | 87.33, 80.50, 75.31 |
Refinement procedure
| Resolution | 37.020 - 1.600 |
| R-factor | 0.175 |
| Rwork | 0.175 |
| R-free | 0.19100 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.309 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 56952 | |
| <I/σ(I)> | 22.9 | 14.1 |
| Completeness [%] | 92.4 | 89.4 |
| Redundancy | 2.6 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch/microseeding | 5 | 298 | Ammonium sulfate, succinate, sodium carbonate, pH 5.0, batch/microseeding, temperature 298K |
