2I3Y
Crystal structure of human glutathione peroxidase 5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8983 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 104.544, 104.544, 71.663 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.17718 |
| Rwork | 0.175 |
| R-free | 0.22835 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f8a |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.458 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.064 | 0.417 |
| Number of reflections | 16114 | |
| <I/σ(I)> | 23 | 5.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.6 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 20% PEG 3350, 0.2 M sodium formate, 5% ETHYLENE GLYCOL, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
