2I3T
Bub3 complex with Mad3 (BubR1) GLEBS motif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2004-10-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.18070 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.352, 173.335, 90.019 |
Unit cell angles | 90.00, 95.30, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.800 |
R-factor | 0.242 |
Rwork | 0.238 |
R-free | 0.26730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u4c |
RMSD bond length | 0.008 |
RMSD bond angle | 1.512 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.367 | |
Number of reflections | 40970 | |
<I/σ(I)> | 1.9 | |
Completeness [%] | 95.2 | 76.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 17% PEG 4K 10% isopropanol 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |