2I36
Crystal structure of trigonal crystal form of ground-state rhodopsin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2004-04-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97970 |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 159.866, 159.866, 142.150 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 4.100 |
| R-factor | 0.38367 |
| Rwork | 0.382 |
| R-free | 0.41153 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hzx |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.327 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.150 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.087 | |
| Number of reflections | 17705 | |
| <I/σ(I)> | 21.1 | |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.3 | 278 | 80 mM MES, pH 6.3, 110 mM N-nonyl-beta-D-glucoside, 200 mM zinc acetate, 0.1% sodium azide, 16 mM beta-mercaptoethanol, 2.6% Merpol HCS, equilibrated against 3.1-3.3 M ammonium sulfate, VAPOR DIFFUSION, temperature 278K |
