2I36
Crystal structure of trigonal crystal form of ground-state rhodopsin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2004-04-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97970 |
Spacegroup name | P 31 1 2 |
Unit cell lengths | 159.866, 159.866, 142.150 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 4.100 |
R-factor | 0.38367 |
Rwork | 0.382 |
R-free | 0.41153 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hzx |
RMSD bond length | 0.011 |
RMSD bond angle | 1.327 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 4.150 |
High resolution limit [Å] | 4.000 | 4.000 |
Rmerge | 0.087 | |
Number of reflections | 17705 | |
<I/σ(I)> | 21.1 | |
Completeness [%] | 99.7 | 100 |
Redundancy | 10.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.3 | 278 | 80 mM MES, pH 6.3, 110 mM N-nonyl-beta-D-glucoside, 200 mM zinc acetate, 0.1% sodium azide, 16 mM beta-mercaptoethanol, 2.6% Merpol HCS, equilibrated against 3.1-3.3 M ammonium sulfate, VAPOR DIFFUSION, temperature 278K |