2I33
The structure of the Class C acid phosphatase from Bacillus anthracis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 140 |
Detector technology | CCD |
Collection date | 2005-10-14 |
Detector | NOIR-1 |
Wavelength(s) | 1.2037 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.231, 89.909, 104.238 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.120 - 1.570 |
R-factor | 0.175 |
Rwork | 0.173 |
R-free | 0.20700 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.318 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.120 | 1.660 |
High resolution limit [Å] | 1.570 | 1.570 |
Number of reflections | 66821 | |
Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.1 M HEPES pH 7.0 30% v/v Jeffamine ED-2001 reagent, VAPOR DIFFUSION, SITTING DROP, temperature 298K |