2I2F
Crystal structure of LmNADK1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.934 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 62.428, 76.361, 119.202 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.140 - 1.900 |
R-factor | 0.206 |
Rwork | 0.205 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2i29 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.403 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.140 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.071 | 0.399 |
Number of reflections | 21902 | |
<I/σ(I)> | 20.9 | 4.1 |
Completeness [%] | 99.0 | 94 |
Redundancy | 6.5 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 0.3 M potassium chloride, 50 mM tri-sodium citrate dihydrate, 15-20% w/v polyethylene glycol 400, pH 5.4, VAPOR DIFFUSION, HANGING DROP |