2I27
Crystal Structure Analysis of the Nurse Shark New Antigen Receptor Ancestral variable domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979454 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 60.423, 61.398, 103.849 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.920 |
| R-factor | 0.21996 |
| Rwork | 0.217 |
| R-free | 0.28052 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.778 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Number of reflections | 15147 | |
| <I/σ(I)> | 33.7 | 4.8 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.9 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 26% PEG 4000, 0.1M hepes, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
