2I1Y
Crystal structure of the phosphatase domain of human PTP IA-2
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.93971 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 72.907, 74.229, 121.067 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 19.730 - 2.230 |
| R因子 | 0.20123 |
| Rwork | 0.199 |
| R-free | 0.26698 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fh7 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.008 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.146 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.340 |
| 分解能 [Å] (高) | 2.230 | 2.230 |
| Rmerge_l_obs | 0.225 | |
| 独立反射数 | 32726 | |
| <I/σ(I)> | 2.7 | 0.5 |
| 完全性 [%] | 98.7 | 98.7 |
| 冗長性 | 6.8 | 5.9 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 294 | 25% PEG3350, 100 MM BIS-TRIS, 200 MM AMMONIUM ACETATE, PH 6.0, 10% GLYCEROL, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
