2I1S
Crystal Structure of Protein of Unknown Function MM3350 from Methanosarcina mazei Go1
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97936, 0.97953 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.421, 90.577, 107.978 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.300 |
R-factor | 0.19456 |
Rwork | 0.192 |
R-free | 0.24284 |
Structure solution method | MAD |
RMSD bond length | 0.019 |
RMSD bond angle | 1.613 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.095 | 0.416 |
Number of reflections | 26070 | |
<I/σ(I)> | 19.2 | |
Completeness [%] | 93.5 | 59.7 |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1 M Sodium citrate 20% isopropanol 20% PEG 4000 , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |