2I13
Aart, a six finger zinc finger designed to recognize ANN triplets
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL1-5 |
Synchrotron site | SSRL |
Beamline | BL1-5 |
Temperature [K] | 100 |
Wavelength(s) | 1.38, 1.2834, 1.2830, 1.2574 |
Spacegroup name | P 1 |
Unit cell lengths | 41.586, 72.054, 74.212 |
Unit cell angles | 75.53, 83.80, 72.67 |
Refinement procedure
Resolution | 50.000 - 1.960 |
R-factor | 0.203 |
Rwork | 0.203 |
R-free | 0.24900 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.713 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 1.960 | 1.960 |
Rmerge | 0.052 | 0.364 |
Number of reflections | 51971 | |
<I/σ(I)> | 37 | 2.3 |
Completeness [%] | 90.5 | 91.3 |
Redundancy | 3.3 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 290 | 20% PEG 4000, 400mM NaCl, 100mM Ammonium Acetate, 100mM citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 4000 | ||
10 | 1 | 2 | HOH | ||
2 | 1 | 1 | NaCl | ||
3 | 1 | 1 | Ammonium Acetate | ||
4 | 1 | 1 | citrate | ||
5 | 1 | 1 | HOH | ||
6 | 1 | 2 | PEG 4000 | ||
7 | 1 | 2 | NaCl | ||
8 | 1 | 2 | Ammonium Acetate | ||
9 | 1 | 2 | citrate |