2I0W
Crystal structure analysis of NP24-I, a thaumatin-like protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 2005-04-07 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 |
Unit cell lengths | 61.010, 61.010, 62.895 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.830 - 2.500 |
R-factor | 0.171 |
Rwork | 0.171 |
R-free | 0.22300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pcv |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | AUTOMAR |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.510 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.114 | 0.337 |
Number of reflections | 8574 | |
<I/σ(I)> | 3.9 | 1.5 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 4.06 | 4.04 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | 0.1M lithium chloride, 20% PEG 3350, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |