2I0I
X-ray crystal structure of Sap97 PDZ3 bound to the C-terminal peptide of HPV18 E6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-05-07 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.167, 61.919, 57.142 |
| Unit cell angles | 90.00, 123.33, 90.00 |
Refinement procedure
| Resolution | 19.970 - 2.800 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.800 |
| Number of reflections | 6381 |
| Completeness [%] | 93.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 291 | PEG4000, 0.1M MES, pH 6.5, VAPOR DIFFUSION, temperature 291K |
