2I05
Escherichia Coli Replication Terminator Protein (Tus) Complexed With TerA DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 63.373, 63.373, 252.652 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.600 |
| R-factor | 0.23029 |
| Rwork | 0.226 |
| R-free | 0.31240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ewj |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.145 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.088 | 0.259 |
| Number of reflections | 13969 | |
| <I/σ(I)> | 28.36 | 3.1 |
| Completeness [%] | 82.0 | 25.5 |
| Redundancy | 5.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 12.5% PEG 3350, 50mM Bis-Tris pH6.5, 200mM NaI, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 3350 | ||
| 2 | 1 | 1 | sodium iodide | ||
| 3 | 1 | 1 | Bis-Tris | ||
| 4 | 1 | 1 | water | ||
| 5 | 1 | 2 | PEG 3350 | ||
| 6 | 1 | 2 | sodium iodide |
