2I04
X-ray crystal structure of MAGI-1 PDZ1 bound to the C-terminal peptide of HPV18 E6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-05-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.961, 27.611, 59.525 |
| Unit cell angles | 90.00, 105.42, 90.00 |
Refinement procedure
| Resolution | 28.690 - 2.150 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.040 |
| RMSD bond angle | 2.800 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 9305 | |
| Completeness [%] | 99.4 | 94.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 291 | PEG2000, 0.1 M Na Acetate , pH 4.6, VAPOR DIFFUSION, temperature 291K |
