2I00
Crystal structure of acetyltransferase (GNAT family) from Enterococcus faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97980 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.410, 177.091, 104.429 |
| Unit cell angles | 90.00, 94.89, 90.00 |
Refinement procedure
| Resolution | 41.920 - 2.300 |
| R-factor | 0.21582 |
| Rwork | 0.213 |
| R-free | 0.26977 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.450 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.100 | 0.700 |
| Number of reflections | 116355 | |
| <I/σ(I)> | 13.06 | 1 |
| Completeness [%] | 98.8 | 89.7 |
| Redundancy | 4.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.2M Tri-sodium citrate dihydrate, 20% PEG3350, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
