2HZR

Crystal structure of human apolipoprotein D (ApoD)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2005-07-11
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97940,0.97969,0.96863
Spacegroup name	P 43 21 2
Unit cell lengths	49.247, 49.247, 143.486
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.800
R-factor	0.205
Rwork	0.202
R-free	0.25000
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.431
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.580	49.270	1.900
High resolution limit [Å]	1.800	5.690	1.800
Rmerge	0.067	0.051	0.285
Total number of observations		7194	35742
Number of reflections	17002
<I/σ(I)>	6.4	12	2.2
Completeness [%]	99.1	95.2	98.7
Redundancy	15	12	14.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	1.1 M lithium sulfate, 0.1 M ammonium formate, 0.075 M Hepes/NaOH, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	1.1 M lithium sulfate, 0.1 M ammonium formate, 0.075 M Hepes/NaOH, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	1.1 M lithium sulfate, 0.1 M ammonium formate, 0.075 M Hepes/NaOH, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K