2HZH
Crystal structure of laccase from Coriolus zonatus at 2.6 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-06 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.05 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 168.930, 168.930, 69.350 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 145.860 - 2.600 |
R-factor | 0.21272 |
Rwork | 0.211 |
R-free | 0.23792 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.170 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 145.860 | 2.620 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 35011 | |
Completeness [%] | 1.0 | 95.2 |
Redundancy | 5.02 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | The crystallizing solution (volume 6 ml) contained the protein at a concentration of 8 mg/ml in 50 mM sodium citrate, 0.1 M ammonium sulfate, and 12.5% (w/v) PEG 4000 in 0.05M sodium acetate buffer at pH 4.6., pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |