2HZB
X-Ray Crystal Structure of Protein BH3568 from Bacillus halodurans. Northeast Structural Genomics Consortium BhR60.
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9793, 0.9796, 0.9681 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.725, 142.012, 229.562 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.980 - 2.800 |
R-factor | 0.185 |
Rwork | 0.185 |
R-free | 0.23900 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data scaling software | HKL-2000 |
Phasing software | SnB |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.092 | 0.399 |
Number of reflections | 71260 | |
<I/σ(I)> | 13.6 | 3 |
Completeness [%] | 98.5 | 95.1 |
Redundancy | 8.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 277 | 2.4M Sodium malonate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 7.00 |