2HZ9
Crystal structure of Lys12Val/Asn95Val/Cys117Val mutant of human acidic fibroblast growth factor at 1.70 angstrom resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2006-07-17 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 76.280, 95.370, 107.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.580 - 1.700 |
| Rwork | 0.199 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jqz |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.580 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.076 | |
| Number of reflections | 38559 | |
| <I/σ(I)> | 44.78 | |
| Completeness [%] | 90.6 | 99.6 |
| Redundancy | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298.15 | Ammonium Sulfate, Sodium Formate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K |
