2HZ9
Crystal structure of Lys12Val/Asn95Val/Cys117Val mutant of human acidic fibroblast growth factor at 1.70 angstrom resolution.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2006-07-17 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 76.280, 95.370, 107.330 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.580 - 1.700 |
Rwork | 0.199 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jqz |
RMSD bond length | 0.012 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.580 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.076 | |
Number of reflections | 38559 | |
<I/σ(I)> | 44.78 | |
Completeness [%] | 90.6 | 99.6 |
Redundancy | 9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298.15 | Ammonium Sulfate, Sodium Formate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K |