2HYZ
Crystal structure of an 8 repeat consensus TPR superhelix (orthorombic crystal form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-08-16 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.50 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.156, 67.695, 70.785 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
R-factor | 0.217 |
Rwork | 0.213 |
R-free | 0.27000 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.259 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.06) |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
Rmerge | 0.101 | 0.054 | 0.208 |
Number of reflections | 14975 | ||
<I/σ(I)> | 9.2 | ||
Completeness [%] | 99.9 | 99.5 | 99.5 |
Redundancy | 3.4 | 3.5 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5 | 298 | 100 mM Acetate, 25% MPD, 50 mM SmCl3, pH 5.0, VAPOR DIFFUSION, temperature 298K |