2HYX

Structure of the C-terminal domain of DipZ from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-22
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9797
Spacegroup name	P 21 21 21
Unit cell lengths	110.070, 117.915, 123.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.900
Rwork	0.195
R-free	0.21900
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.354
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.06)
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.970
High resolution limit [Å]	1.900	4.090	1.900
Rmerge	0.064	0.033	0.350
Number of reflections	126251
<I/σ(I)>	13
Completeness [%]	99.9	99.8	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	298	20% PEG4000, 0.1M Na-Citrate pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K