2HYV
Human Annexin A2 with heparin hexasaccharide bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-04-01 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 107.709, 54.292, 68.571 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.420 |
Rwork | 0.212 |
R-free | 0.22300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | : 1XJL |
RMSD bond length | 0.004 |
RMSD bond angle | 1.040 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR (2.5) |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.470 |
High resolution limit [Å] | 1.420 | 3.060 | 1.420 |
Rmerge | 0.055 | 0.035 | 0.375 |
Number of reflections | 75286 | ||
<I/σ(I)> | 41.5 | ||
Completeness [%] | 97.9 | 99.5 | 83.5 |
Redundancy | 5 | 7.3 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 5 | 290 | 1,6 hexanediol, pH 5.0, hanging drop, temperature 290K |
1 | hanging drop | 5 | 290 | 1,6 hexanediol, pH 5.0, hanging drop, temperature 290K |