2HYV

Human Annexin A2 with heparin hexasaccharide bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-04-01
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	107.709, 54.292, 68.571
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.420
Rwork	0.212
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	: 1XJL
RMSD bond length	0.004
RMSD bond angle	1.040
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR (2.5)
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.470
High resolution limit [Å]	1.420	3.060	1.420
Rmerge	0.055	0.035	0.375
Number of reflections	75286
<I/σ(I)>	41.5
Completeness [%]	97.9	99.5	83.5
Redundancy	5	7.3	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	5	290	1,6 hexanediol, pH 5.0, hanging drop, temperature 290K
1	hanging drop	5	290	1,6 hexanediol, pH 5.0, hanging drop, temperature 290K