2HYK
The crystal structure of an endo-beta-1,3-glucanase from alkaliphilic Nocardiopsis sp.strain F96
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-11-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 34.589, 71.843, 39.666 |
Unit cell angles | 90.00, 90.21, 90.00 |
Refinement procedure
Resolution | 39.650 - 1.300 |
R-factor | 0.144 |
Rwork | 0.143 |
R-free | 0.16200 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.250 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.08) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.350 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.064 | 0.350 |
Number of reflections | 47558 | |
<I/σ(I)> | 14.2 | |
Completeness [%] | 99.3 | 95.7 |
Redundancy | 6.2 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1M Tris-HCl pH 8.0, 1.3-1.4M Ammonium sulfate, 1% Ethanol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |