2HYJ
The crystal structure of a tetR-family transcriptional regulator from Streptomyces coelicolor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97980 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 32.864, 82.678, 178.933 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.700 - 2.190 |
| R-factor | 0.1939 |
| Rwork | 0.191 |
| R-free | 0.24690 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.289 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.400 | 2.250 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.122 | 0.560 |
| Number of reflections | 12388 | |
| <I/σ(I)> | 18.04 | 2.2 |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 8.9 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M Na Hepes, 0.2M Calcium chloride, 28% PEG400, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
