2HYB
Crystal Structure of Hexameric DsrEFH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.610, 183.110, 107.830 |
| Unit cell angles | 90.00, 99.57, 90.00 |
Refinement procedure
| Resolution | 19.980 - 2.500 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.26200 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 72592 | |
| Completeness [%] | 97.8 | 81.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 20mg/ml protein, 0.1M Bis-Tris pH5.5 0.2M Lithium Sulfate 25% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
