2HY7
Crystal Structure of GumK, a beta-glucuronosyltransferase from Xanthomonas campestris
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-27 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 123.629, 123.629, 174.307 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 107.210 - 1.900 |
R-factor | 0.182 |
Rwork | 0.181 |
R-free | 0.20400 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.277 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 107.211 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.069 | 0.344 |
Number of reflections | 62401 | |
<I/σ(I)> | 9.9 | 2.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 14.1 | 12.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.2 | 293 | 0.1 M Tris-HCl, 35% (w/v) PEG 3350, 0.2 M Li2SO4, 0.1 M CsCl, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 8.20 | |
1 | 8.2 | 293 | 0.1 M Tris-HCl, 35% (w/v) PEG 3350, 0.2 M Li2SO4, 0.1 M CsCl, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 8.20 |