2HY5
Crystal structure of DsrEFH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-11-09 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 98.745, 68.008, 61.957 |
Unit cell angles | 90.00, 122.86, 90.00 |
Refinement procedure
Resolution | 19.670 - 1.720 |
R-factor | 0.182 |
Rwork | 0.182 |
R-free | 0.21100 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 33299 | |
Completeness [%] | 98.1 | 12.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.2M MgCl2, 0.1M Tris-HCl, 10% PEG8000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |