2HY3
Crystal structure of the human tyrosine receptor phosphate gamma in complex with vanadate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-16 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97891 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.173, 74.787, 82.071 |
Unit cell angles | 90.00, 99.58, 90.00 |
Refinement procedure
Resolution | 17.000 - 2.600 |
R-factor | 0.20614 |
Rwork | 0.202 |
R-free | 0.28796 |
Structure solution method | SAD |
RMSD bond length | 0.021 |
RMSD bond angle | 2.054 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 20061 | |
Completeness [%] | 87.0 | 89 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 12% PEG3350, 0.1M Tris, 0.3M NaCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |