2HY3
Crystal structure of the human tyrosine receptor phosphate gamma in complex with vanadate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97891 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.173, 74.787, 82.071 |
| Unit cell angles | 90.00, 99.58, 90.00 |
Refinement procedure
| Resolution | 17.000 - 2.600 |
| R-factor | 0.20614 |
| Rwork | 0.202 |
| R-free | 0.28796 |
| Structure solution method | SAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.054 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 20061 | |
| Completeness [%] | 87.0 | 89 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 12% PEG3350, 0.1M Tris, 0.3M NaCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
