2HXS

Crystal Structure of Rab28A GTPase in the Inactive (GDP-3'P-Bound) Form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MAR scanner 345 mm plate
Spacegroup name	P 21 21 21
Unit cell lengths	30.510, 56.621, 85.109
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.000 - 1.100
R-factor	0.152
Rwork	0.149
R-free	0.18360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yzk
RMSD bond length	0.024
RMSD bond angle	2.184
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.000	1.140
High resolution limit [Å]	1.100	1.100
Rmerge	0.075	0.367
Number of reflections	58782
<I/σ(I)>	24.7	4.25
Completeness [%]	98.3	96.8
Redundancy	8.3	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	20% PEG 3360, 100 mM NaCl, 100 mM Potassium Acetate, 5% glycerol, 20 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K