2HXP

Crystal Structure of the human phosphatase (DUSP9)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-30
Detector	ADSC QUANTUM 210
Wavelength(s)	1.1, 1.7
Spacegroup name	P 32
Unit cell lengths	50.527, 50.527, 59.851
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.700 - 1.830
R-factor	0.216
Rwork	0.216
R-free	0.24600
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.700	1.900
High resolution limit [Å]	1.830	1.830
Rmerge	0.022	0.140
Number of reflections	14920
<I/σ(I)>	47.1	19
Completeness [%]	99.2	100
Redundancy	11.4	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	25% PEG 3350, 0.1M Bis-Tris, 0.2M Ammonium Sulphate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K