2HXO
Structure of the transcriptional regulator SCO7222, a TetR from Streptomyces coelicolor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97980 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.088, 97.479, 100.971 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.940 - 2.400 |
R-factor | 0.226 |
Rwork | 0.226 |
R-free | 0.26000 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXCD |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 45734 | |
<I/σ(I)> | 31.1 | 1.8 |
Completeness [%] | 99.8 | 99.6 |
Redundancy | 8.7 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 3% MPD, 5% Isopropanol, 35 mM Magnesium chloride, 30 mM Potassium chloride and 500 mM Sodium cacodylate, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |