2HXI
Structural Genomics, the crystal structure of a putative transcriptional regulator from Streptomyces coelicolor A3(2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-21 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.338, 94.868, 51.835 |
| Unit cell angles | 90.00, 95.61, 90.00 |
Refinement procedure
| Resolution | 22.610 - 1.700 |
| R-factor | 0.18964 |
| Rwork | 0.187 |
| R-free | 0.23187 |
| Structure solution method | SAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.828 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKLSBC |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.610 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.097 | 0.478 |
| Number of reflections | 46555 | |
| <I/σ(I)> | 23.03 | 2.05 |
| Completeness [%] | 98.7 | 90.8 |
| Redundancy | 3.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 283 | 0.2M NH4Cl, 20% PEG3350, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 283K |
