2HWU
Crystal structure of the uridine phosphorylase from Salmonella typhimurium in complex with uridine and phosphate ion at 2.91A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8048 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.880, 123.990, 134.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 90.910 - 2.910 |
| R-factor | 0.21074 |
| Rwork | 0.208 |
| R-free | 0.25594 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.003 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.910 | 2.985 |
| High resolution limit [Å] | 2.910 | 2.909 |
| Number of reflections | 30842 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 297 | VAPOR DIFFUSION, HANGING DROP, temperature 297K |
