2HWJ
Crystal structure of protein Atu1540 from Agrobacterium tumefaciens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97932 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 132.079, 173.749, 142.077 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.610 |
| R-factor | 0.20183 |
| Rwork | 0.198 |
| R-free | 0.27261 |
| Structure solution method | SAD |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.329 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.133 | 0.679 |
| Number of reflections | 49478 | |
| <I/σ(I)> | 34 | 1.5 |
| Completeness [%] | 99.1 | 91.7 |
| Redundancy | 9.9 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1M Tris-HCl, 0.01M Magnesium chloride, 22% PEG400, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
