2HW4
Crystal structure of human phosphohistidine phosphatase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.033130 |
| Spacegroup name | H 3 |
| Unit cell lengths | 112.530, 112.530, 29.370 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.270 - 1.900 |
| R-factor | 0.17325 |
| Rwork | 0.171 |
| R-free | 0.22469 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Se-Met MAD structure determined from other crystals |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.774 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 10665 | |
| <I/σ(I)> | 20.62 | 6.47 |
| Completeness [%] | 97.5 | 88.8 |
| Redundancy | 5.6 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 1.9 M Sodium Formate, 0.1 M Bis-tris propane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
