2HVQ

Structure of Adenylated full-length T4 RNA Ligase 2

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-04-07
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
格子定数 [Å]	48.020, 57.850, 119.490
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	41.560 - 2.400
Rwork	0.211
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1s68
結合長の平均二乗偏差(RMSD) [Å]	0.006
結合角の平均二乗偏差(RMSD) [度]	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	41.560	2.490
分解能 [Å] (高)	2.400	2.400
Rmerge_l_obs	0.120	0.382
独立反射数	12520
<I/σ(I)>	7.4	3.2
完全性 [%]	91.6	89.6
冗長性	3.3	3.2

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	5.75	291	100 mM MES, 10% MPD, 30 mM NaCl, pH 5.75, VAPOR DIFFUSION, HANGING DROP, temperature 291K