2HVH
ddCTP:O6MeG pair in the polymerase active site (0 position)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-07 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 94.060, 109.390, 151.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.298 - 2.492 |
Rwork | 0.201 |
R-free | 0.26200 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2hhw |
RMSD bond length | 0.008 |
RMSD bond angle | 1.269 |
Data reduction software | XDS |
Data scaling software | SCALA |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.298 | 2.630 |
High resolution limit [Å] | 2.492 | 2.492 |
Rmerge | 0.135 | 0.414 |
Total number of observations | 36059 | |
Number of reflections | 54569 | |
<I/σ(I)> | 11.4 | 3.2 |
Completeness [%] | 98.8 | 93.3 |
Redundancy | 5.6 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 290 | 55% saturated ammonium sulfate, 100 mM MES, 2.5% MPD, 10mM MnSO4, 10mM MgSO4, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | ammonium sulfate | ||
10 | 1 | 2 | H2O | ||
2 | 1 | 1 | MPD | ||
3 | 1 | 1 | MES | ||
4 | 1 | 1 | MnSO4 | ||
5 | 1 | 1 | MgSO4 | ||
6 | 1 | 1 | H2O | ||
7 | 1 | 2 | MnSO4 | ||
8 | 1 | 2 | MgSO4 | ||
9 | 1 | 2 | ammonium sulfate |