2HVH
ddCTP:O6MeG pair in the polymerase active site (0 position)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 94.060, 109.390, 151.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.298 - 2.492 |
| Rwork | 0.201 |
| R-free | 0.26200 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2hhw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.269 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.298 | 2.630 |
| High resolution limit [Å] | 2.492 | 2.492 |
| Rmerge | 0.135 | 0.414 |
| Total number of observations | 36059 | |
| Number of reflections | 54569 | |
| <I/σ(I)> | 11.4 | 3.2 |
| Completeness [%] | 98.8 | 93.3 |
| Redundancy | 5.6 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 290 | 55% saturated ammonium sulfate, 100 mM MES, 2.5% MPD, 10mM MnSO4, 10mM MgSO4, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ammonium sulfate | ||
| 10 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MES | ||
| 4 | 1 | 1 | MnSO4 | ||
| 5 | 1 | 1 | MgSO4 | ||
| 6 | 1 | 1 | H2O | ||
| 7 | 1 | 2 | MnSO4 | ||
| 8 | 1 | 2 | MgSO4 | ||
| 9 | 1 | 2 | ammonium sulfate |
