2HVF
Crystal Structure of N-terminal Domain of Ribosomal Protein L9 (NTL9), G34dA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 53.517, 53.517, 36.178 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.570 |
| R-factor | 0.162 |
| Rwork | 0.159 |
| R-free | 0.20800 |
| Structure solution method | Phase from wild type NTL9 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.622 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.062 | 0.286 |
| Number of reflections | 7705 | |
| <I/σ(I)> | 15.5 | |
| Completeness [%] | 99.2 | 92.8 |
| Redundancy | 9.9 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 10 mM Imidazole (pH 8.0), 300 mM Zn Acetate, 3 M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
