2HVD

Human nucleoside diphosphate kinase A complexed with ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-03-07
Detector	MARRESEARCH
Wavelength(s)	0.973
Spacegroup name	P 41 21 2
Unit cell lengths	114.260, 114.260, 89.789
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.650 - 2.150
R-factor	0.24911
Rwork	0.247
R-free	0.29374
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ucn
RMSD bond length	0.010
RMSD bond angle	1.299
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Refinement software	REFMAC (5.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.650	2.270
High resolution limit [Å]	2.150	2.150
Number of reflections	30827
<I/σ(I)>	10.2	3.1
Completeness [%]	97.2	99
Redundancy	3.2	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	2.4 M AMMONIUM SULPHATE,10 MM ADP, 20 MM MGCL2, 4 MM DTT, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K