2HV2
Crystal Structure of Conserved Protein of Unknown Function from Enterococcus faecalis V583 at 2.4 A Resolution, Probable N-Acyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97929 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 189.626, 104.277, 152.281 |
| Unit cell angles | 90.00, 98.26, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.18751 |
| Rwork | 0.185 |
| R-free | 0.23347 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.404 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.095 | 0.480 |
| Number of reflections | 112275 | |
| <I/σ(I)> | 9.43 | 1.23 |
| Completeness [%] | 97.5 | 87.4 |
| Redundancy | 4 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 298 | pH 6.0, VAPOR DIFFUSION, temperature 298K |
| 1 | VAPOR DIFFUSION | 6 | 298 | pH 6.0, VAPOR DIFFUSION, temperature 298K |
