2HUZ
Crystal structure of GNPNAT1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-07-23 |
| Detector | RIGAKU |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.668, 47.645, 84.843 |
| Unit cell angles | 90.00, 123.52, 90.00 |
Refinement procedure
| Resolution | 70.710 - 2.670 |
| R-factor | 0.2048 |
| Rwork | 0.200 |
| R-free | 0.29841 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.313 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.710 | 2.800 |
| High resolution limit [Å] | 2.670 | 2.670 |
| Number of reflections | 10107 | |
| Completeness [%] | 96.5 | 94.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 300 | 25% PEG4K, 0.2M NH4SO4, 0.1M Na Acetate pH4.6, 0.1M Yttrium Chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
