2HUP

Crystal structure of human RAB43 in complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-06-27
Detector	RIGAKU RAXIS
Wavelength(s)	1.54178
Spacegroup name	P 32 2 1
Unit cell lengths	66.447, 66.447, 145.116
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.050
Rwork	0.233
R-free	0.28620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ew1
RMSD bond length	0.015
RMSD bond angle	1.410
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.120
High resolution limit [Å]	2.050	4.410	2.050
Rmerge	0.116	0.054	0.955
Number of reflections	24106	2578	2335
<I/σ(I)>	6.7
Completeness [%]	99.9
Redundancy	9.6	9.5	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	25% PEG8000, 0.2M NaCl, 0.1M Tris-HCl, pH 8.5, vapor diffusion, sitting drop, temperature 291K, VAPOR DIFFUSION, SITTING DROP
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	25% PEG8000, 0.2M NaCl, 0.1M Tris-HCl, pH 8.5, vapor diffusion, sitting drop, temperature 291K, VAPOR DIFFUSION, SITTING DROP