2HUM
Crystal structure of T4 Lysozyme D72C synthetic dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-02-02 |
| Detector | RIGAKU |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.306, 55.907, 80.292 |
| Unit cell angles | 90.00, 108.13, 90.00 |
Refinement procedure
| Resolution | 52.600 - 2.350 |
| R-factor | 0.24277 |
| Rwork | 0.239 |
| R-free | 0.30355 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c6t |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.080 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.250 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 27769 | |
| Completeness [%] | 99.5 | 98.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.2 M tri-Lithium Citrate, 20% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
