2HUL
Crystal structure of T4 Lysozyme S44C synthetic dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-05-26 |
| Detector | RIGAKU |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.598, 28.926, 88.814 |
| Unit cell angles | 90.00, 119.42, 90.00 |
Refinement procedure
| Resolution | 40.320 - 1.800 |
| R-factor | 0.179 |
| Rwork | 0.178 |
| R-free | 0.19200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c6t |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.359 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.380 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 19413 | |
| <I/σ(I)> | 17.6 | 4.2 |
| Completeness [%] | 99.1 | 94.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.7 | 298 | 2.0 M Ammonium Sulfate, 0.1 M Cacodylate pH 6.7, 0.2 M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 6.70 |
