2HUB
Structure of Hen Egg-White Lysozyme Determined from crystals grown in pH 7.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-16 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9737 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 76.861, 76.861, 35.867 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.310 - 1.201 |
R-factor | 0.138 |
Rwork | 0.137 |
R-free | 0.17300 |
Structure solution method | MAD |
Starting model (for MR) | In-house structure |
RMSD bond length | 0.014 |
RMSD bond angle | 1.697 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | REFMAC (5.2) |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.310 | 1.220 |
High resolution limit [Å] | 1.201 | 1.201 |
Rmerge | 0.054 | 0.475 |
Number of reflections | 33587 | |
<I/σ(I)> | 16.8 | 2.1 |
Completeness [%] | 98.2 | 74.3 |
Redundancy | 6.2 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbatch, Douglas Instrument robot | 7.5 | 293 | 0.1 M HEPES-Na pH 7.5, 0.8M Potassium Sodium Tartrate tetrahydrate, Microbatch, Douglas Instrument robot, temperature 293K |