2HU8
Binding of inhibitors by Acylaminoacyl peptidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-10-21 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.810, 103.922, 169.255 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 2.400 |
| R-factor | 0.21778 |
| Rwork | 0.215 |
| R-free | 0.26128 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.878 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.960 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.137 | 0.380 |
| Number of reflections | 45009 | |
| <I/σ(I)> | 11.68 | 4.46 |
| Completeness [%] | 99.1 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 20mM Tris/HCl, 20% ethanol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
