2HTX
Crystal Structure Analysis of Hen Egg White Lysozyme Crosslinked by Polymerized Glutaraldehyde in Acidic Environment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.931 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.856, 78.856, 36.954 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.730 - 1.560 |
| R-factor | 0.12937 |
| Rwork | 0.127 |
| R-free | 0.18240 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | In-House Coordinates |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.445 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC (5.2) |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.730 | 1.590 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Rmerge | 0.036 | 0.367 |
| Number of reflections | 17157 | |
| <I/σ(I)> | 47.2 | 2.34 |
| Completeness [%] | 97.7 | 87.1 |
| Redundancy | 7.5 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 291 | 0.1M Sodium Acetate, 7% NaCl, 25% Ethylene glycol, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
